96 well applied biosystem micro amp fast optical reaction plate Search Results


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Thermo Fisher 3730 xl 96 capillary dna analyzer
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Promega 96 well veritas luminometer
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Axion BioSystems cytoview mea plate
Characterization of cardiomyocytes-derived from human pluripotent stem <t>cells</t> <t>(hPSC-CMs).</t> (A) Schematic diagram of hPSC differentiation into cardiomyocytes. (B) The morphology of differentiated hPSC-CMs derived from two hPSC cell lines (hESCs: H9 and hiPSCs: CMC-11; 10 × magnification). (C) The RT-qPCR was performed to measure the expression of cardiac sarcomeric markers, cTnT, and MYH6 genes, in hPSC-CMs. The data represent the mean (±SD) of at least two independent experiments performed in triplicate. Statistical analyses were determined using paired student's t -tests, and P < 0.05 was considered as significant (*). (D) Expression of TNNT2 and ACTN2 in hPSC-CMs (hESC-CMs [upper panel] and hiPSC-CMs [lower panel]) at day 15 of differentiation were determined by flow cytometry and immunofluorescence analyses. Scale bar, 100 μm. (E) Beating cardiomyocytes were prepared for action potential (AP) recording using the patch-clamp technique in hESC-CMs and field potential (FP) recording using a multielectrode array <t>(MEA)</t> system in hiPSC-CMs. The hPSC-CMs were treated with 100 nM isoprenaline (β 2 -adrenergic receptor agonist), 0.3 μM and 1 μM nifedipine (L-type Ca 2+ channel blocker), and 10 nM dofetilide and 30 nM E4031 (hERG K + channel blockers).
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Promega celltiter 96® aqueous nonradioactive cell proliferation assay kit
Characterization of cardiomyocytes-derived from human pluripotent stem <t>cells</t> <t>(hPSC-CMs).</t> (A) Schematic diagram of hPSC differentiation into cardiomyocytes. (B) The morphology of differentiated hPSC-CMs derived from two hPSC cell lines (hESCs: H9 and hiPSCs: CMC-11; 10 × magnification). (C) The RT-qPCR was performed to measure the expression of cardiac sarcomeric markers, cTnT, and MYH6 genes, in hPSC-CMs. The data represent the mean (±SD) of at least two independent experiments performed in triplicate. Statistical analyses were determined using paired student's t -tests, and P < 0.05 was considered as significant (*). (D) Expression of TNNT2 and ACTN2 in hPSC-CMs (hESC-CMs [upper panel] and hiPSC-CMs [lower panel]) at day 15 of differentiation were determined by flow cytometry and immunofluorescence analyses. Scale bar, 100 μm. (E) Beating cardiomyocytes were prepared for action potential (AP) recording using the patch-clamp technique in hESC-CMs and field potential (FP) recording using a multielectrode array <t>(MEA)</t> system in hiPSC-CMs. The hPSC-CMs were treated with 100 nM isoprenaline (β 2 -adrenergic receptor agonist), 0.3 μM and 1 μM nifedipine (L-type Ca 2+ channel blocker), and 10 nM dofetilide and 30 nM E4031 (hERG K + channel blockers).
Celltiter 96® Aqueous Nonradioactive Cell Proliferation Assay Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Thermo Fisher 384 well plate
Characterization of cardiomyocytes-derived from human pluripotent stem <t>cells</t> <t>(hPSC-CMs).</t> (A) Schematic diagram of hPSC differentiation into cardiomyocytes. (B) The morphology of differentiated hPSC-CMs derived from two hPSC cell lines (hESCs: H9 and hiPSCs: CMC-11; 10 × magnification). (C) The RT-qPCR was performed to measure the expression of cardiac sarcomeric markers, cTnT, and MYH6 genes, in hPSC-CMs. The data represent the mean (±SD) of at least two independent experiments performed in triplicate. Statistical analyses were determined using paired student's t -tests, and P < 0.05 was considered as significant (*). (D) Expression of TNNT2 and ACTN2 in hPSC-CMs (hESC-CMs [upper panel] and hiPSC-CMs [lower panel]) at day 15 of differentiation were determined by flow cytometry and immunofluorescence analyses. Scale bar, 100 μm. (E) Beating cardiomyocytes were prepared for action potential (AP) recording using the patch-clamp technique in hESC-CMs and field potential (FP) recording using a multielectrode array <t>(MEA)</t> system in hiPSC-CMs. The hPSC-CMs were treated with 100 nM isoprenaline (β 2 -adrenergic receptor agonist), 0.3 μM and 1 μM nifedipine (L-type Ca 2+ channel blocker), and 10 nM dofetilide and 30 nM E4031 (hERG K + channel blockers).
384 Well Plate, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Bio-Rad cfx 96
Characterization of cardiomyocytes-derived from human pluripotent stem <t>cells</t> <t>(hPSC-CMs).</t> (A) Schematic diagram of hPSC differentiation into cardiomyocytes. (B) The morphology of differentiated hPSC-CMs derived from two hPSC cell lines (hESCs: H9 and hiPSCs: CMC-11; 10 × magnification). (C) The RT-qPCR was performed to measure the expression of cardiac sarcomeric markers, cTnT, and MYH6 genes, in hPSC-CMs. The data represent the mean (±SD) of at least two independent experiments performed in triplicate. Statistical analyses were determined using paired student's t -tests, and P < 0.05 was considered as significant (*). (D) Expression of TNNT2 and ACTN2 in hPSC-CMs (hESC-CMs [upper panel] and hiPSC-CMs [lower panel]) at day 15 of differentiation were determined by flow cytometry and immunofluorescence analyses. Scale bar, 100 μm. (E) Beating cardiomyocytes were prepared for action potential (AP) recording using the patch-clamp technique in hESC-CMs and field potential (FP) recording using a multielectrode array <t>(MEA)</t> system in hiPSC-CMs. The hPSC-CMs were treated with 100 nM isoprenaline (β 2 -adrenergic receptor agonist), 0.3 μM and 1 μM nifedipine (L-type Ca 2+ channel blocker), and 10 nM dofetilide and 30 nM E4031 (hERG K + channel blockers).
Cfx 96, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Thermo Fisher 6 carboxyfluorescein fam
Characterization of cardiomyocytes-derived from human pluripotent stem <t>cells</t> <t>(hPSC-CMs).</t> (A) Schematic diagram of hPSC differentiation into cardiomyocytes. (B) The morphology of differentiated hPSC-CMs derived from two hPSC cell lines (hESCs: H9 and hiPSCs: CMC-11; 10 × magnification). (C) The RT-qPCR was performed to measure the expression of cardiac sarcomeric markers, cTnT, and MYH6 genes, in hPSC-CMs. The data represent the mean (±SD) of at least two independent experiments performed in triplicate. Statistical analyses were determined using paired student's t -tests, and P < 0.05 was considered as significant (*). (D) Expression of TNNT2 and ACTN2 in hPSC-CMs (hESC-CMs [upper panel] and hiPSC-CMs [lower panel]) at day 15 of differentiation were determined by flow cytometry and immunofluorescence analyses. Scale bar, 100 μm. (E) Beating cardiomyocytes were prepared for action potential (AP) recording using the patch-clamp technique in hESC-CMs and field potential (FP) recording using a multielectrode array <t>(MEA)</t> system in hiPSC-CMs. The hPSC-CMs were treated with 100 nM isoprenaline (β 2 -adrenergic receptor agonist), 0.3 μM and 1 μM nifedipine (L-type Ca 2+ channel blocker), and 10 nM dofetilide and 30 nM E4031 (hERG K + channel blockers).
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Axion BioSystems cytoview-z
Characterization of cardiomyocytes-derived from human pluripotent stem <t>cells</t> <t>(hPSC-CMs).</t> (A) Schematic diagram of hPSC differentiation into cardiomyocytes. (B) The morphology of differentiated hPSC-CMs derived from two hPSC cell lines (hESCs: H9 and hiPSCs: CMC-11; 10 × magnification). (C) The RT-qPCR was performed to measure the expression of cardiac sarcomeric markers, cTnT, and MYH6 genes, in hPSC-CMs. The data represent the mean (±SD) of at least two independent experiments performed in triplicate. Statistical analyses were determined using paired student's t -tests, and P < 0.05 was considered as significant (*). (D) Expression of TNNT2 and ACTN2 in hPSC-CMs (hESC-CMs [upper panel] and hiPSC-CMs [lower panel]) at day 15 of differentiation were determined by flow cytometry and immunofluorescence analyses. Scale bar, 100 μm. (E) Beating cardiomyocytes were prepared for action potential (AP) recording using the patch-clamp technique in hESC-CMs and field potential (FP) recording using a multielectrode array <t>(MEA)</t> system in hiPSC-CMs. The hPSC-CMs were treated with 100 nM isoprenaline (β 2 -adrenergic receptor agonist), 0.3 μM and 1 μM nifedipine (L-type Ca 2+ channel blocker), and 10 nM dofetilide and 30 nM E4031 (hERG K + channel blockers).
Cytoview Z, supplied by Axion BioSystems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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SRU Biosystems 96-well sru bindtm streptavidin-coated plates
Characterization of cardiomyocytes-derived from human pluripotent stem <t>cells</t> <t>(hPSC-CMs).</t> (A) Schematic diagram of hPSC differentiation into cardiomyocytes. (B) The morphology of differentiated hPSC-CMs derived from two hPSC cell lines (hESCs: H9 and hiPSCs: CMC-11; 10 × magnification). (C) The RT-qPCR was performed to measure the expression of cardiac sarcomeric markers, cTnT, and MYH6 genes, in hPSC-CMs. The data represent the mean (±SD) of at least two independent experiments performed in triplicate. Statistical analyses were determined using paired student's t -tests, and P < 0.05 was considered as significant (*). (D) Expression of TNNT2 and ACTN2 in hPSC-CMs (hESC-CMs [upper panel] and hiPSC-CMs [lower panel]) at day 15 of differentiation were determined by flow cytometry and immunofluorescence analyses. Scale bar, 100 μm. (E) Beating cardiomyocytes were prepared for action potential (AP) recording using the patch-clamp technique in hESC-CMs and field potential (FP) recording using a multielectrode array <t>(MEA)</t> system in hiPSC-CMs. The hPSC-CMs were treated with 100 nM isoprenaline (β 2 -adrenergic receptor agonist), 0.3 μM and 1 μM nifedipine (L-type Ca 2+ channel blocker), and 10 nM dofetilide and 30 nM E4031 (hERG K + channel blockers).
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Curiox Biosystems laminar wash 96-well plate
Characterization of cardiomyocytes-derived from human pluripotent stem <t>cells</t> <t>(hPSC-CMs).</t> (A) Schematic diagram of hPSC differentiation into cardiomyocytes. (B) The morphology of differentiated hPSC-CMs derived from two hPSC cell lines (hESCs: H9 and hiPSCs: CMC-11; 10 × magnification). (C) The RT-qPCR was performed to measure the expression of cardiac sarcomeric markers, cTnT, and MYH6 genes, in hPSC-CMs. The data represent the mean (±SD) of at least two independent experiments performed in triplicate. Statistical analyses were determined using paired student's t -tests, and P < 0.05 was considered as significant (*). (D) Expression of TNNT2 and ACTN2 in hPSC-CMs (hESC-CMs [upper panel] and hiPSC-CMs [lower panel]) at day 15 of differentiation were determined by flow cytometry and immunofluorescence analyses. Scale bar, 100 μm. (E) Beating cardiomyocytes were prepared for action potential (AP) recording using the patch-clamp technique in hESC-CMs and field potential (FP) recording using a multielectrode array <t>(MEA)</t> system in hiPSC-CMs. The hPSC-CMs were treated with 100 nM isoprenaline (β 2 -adrenergic receptor agonist), 0.3 μM and 1 μM nifedipine (L-type Ca 2+ channel blocker), and 10 nM dofetilide and 30 nM E4031 (hERG K + channel blockers).
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Image Search Results


Characterization of cardiomyocytes-derived from human pluripotent stem cells (hPSC-CMs). (A) Schematic diagram of hPSC differentiation into cardiomyocytes. (B) The morphology of differentiated hPSC-CMs derived from two hPSC cell lines (hESCs: H9 and hiPSCs: CMC-11; 10 × magnification). (C) The RT-qPCR was performed to measure the expression of cardiac sarcomeric markers, cTnT, and MYH6 genes, in hPSC-CMs. The data represent the mean (±SD) of at least two independent experiments performed in triplicate. Statistical analyses were determined using paired student's t -tests, and P < 0.05 was considered as significant (*). (D) Expression of TNNT2 and ACTN2 in hPSC-CMs (hESC-CMs [upper panel] and hiPSC-CMs [lower panel]) at day 15 of differentiation were determined by flow cytometry and immunofluorescence analyses. Scale bar, 100 μm. (E) Beating cardiomyocytes were prepared for action potential (AP) recording using the patch-clamp technique in hESC-CMs and field potential (FP) recording using a multielectrode array (MEA) system in hiPSC-CMs. The hPSC-CMs were treated with 100 nM isoprenaline (β 2 -adrenergic receptor agonist), 0.3 μM and 1 μM nifedipine (L-type Ca 2+ channel blocker), and 10 nM dofetilide and 30 nM E4031 (hERG K + channel blockers).

Journal: Antiviral Research

Article Title: Antiviral activity and safety of remdesivir against SARS-CoV-2 infection in human pluripotent stem cell-derived cardiomyocytes

doi: 10.1016/j.antiviral.2020.104955

Figure Lengend Snippet: Characterization of cardiomyocytes-derived from human pluripotent stem cells (hPSC-CMs). (A) Schematic diagram of hPSC differentiation into cardiomyocytes. (B) The morphology of differentiated hPSC-CMs derived from two hPSC cell lines (hESCs: H9 and hiPSCs: CMC-11; 10 × magnification). (C) The RT-qPCR was performed to measure the expression of cardiac sarcomeric markers, cTnT, and MYH6 genes, in hPSC-CMs. The data represent the mean (±SD) of at least two independent experiments performed in triplicate. Statistical analyses were determined using paired student's t -tests, and P < 0.05 was considered as significant (*). (D) Expression of TNNT2 and ACTN2 in hPSC-CMs (hESC-CMs [upper panel] and hiPSC-CMs [lower panel]) at day 15 of differentiation were determined by flow cytometry and immunofluorescence analyses. Scale bar, 100 μm. (E) Beating cardiomyocytes were prepared for action potential (AP) recording using the patch-clamp technique in hESC-CMs and field potential (FP) recording using a multielectrode array (MEA) system in hiPSC-CMs. The hPSC-CMs were treated with 100 nM isoprenaline (β 2 -adrenergic receptor agonist), 0.3 μM and 1 μM nifedipine (L-type Ca 2+ channel blocker), and 10 nM dofetilide and 30 nM E4031 (hERG K + channel blockers).

Article Snippet: For MEA analysis, hPSC-CMs were seeded on 96-well MEA plates (Cytoview MEA plate, Axion BioSystems, USA) prior to drug treatment.

Techniques: Derivative Assay, Quantitative RT-PCR, Expressing, Flow Cytometry, Immunofluorescence, Patch Clamp